HPLC Column, IEX WCX, 5um, 2.1mm ID x 150mm Length, 100A. Cogent Brand. 1 EA.

Additional Information:

Cogent IEX WCX is a weak cation exchange (WCX) HPLC stationary phase designed for the retention and separation of positively charged compounds. Manufactured using high-purity silica and carboxylic acid functional groups, the stationary phase provides a negatively charged surface within its functional pH range of approximately 2.5 to 7, enabling reliable cation exchange selectivity for basic compounds and other positively charged analytes. This chemistry is widely used in ion exchange chromatography and serves as an excellent alternative for many USP L9 methods.

67591-15P-2 is a 150 mm x 2.1 mm ID analytical HPLC column packed with 5 µm WCX particles with a 100 Å pore size. The 150 mm column length provides increased resolving power and peak capacity compared to shorter columns, making it ideal for impurity profiling, complex separations, and method optimization. The 2.1 mm ID format is particularly well suited for LC-MS applications and reduced solvent consumption. Typical flow rates are approximately 0.2–0.3 mL/min; however, standard HPLC systems must be capable of reliable low-flow operation to ensure accurate and reproducible chromatographic performance.

This weak cation exchange column is particularly useful for the analysis and purification of basic pharmaceuticals, amines, peptides, proteins, and other positively charged compounds. The combination of selective WCX functionality, high-purity silica, and efficient 5 µm particles provides excellent chromatographic performance for demanding ion exchange applications requiring enhanced resolution while maintaining LC-MS compatibility and reduced solvent usage.

Key Benefits

  • Weak cation exchange (WCX) stationary phase

  • Carboxylic acid functional groups

  • Suitable for positively charged analytes

  • Alternative for many USP L9 methods

  • High-purity silica support

  • 5 µm particles for efficient separations

  • Enhanced resolution from 150 mm column length

  • Reduced solvent consumption with 2.1 mm ID format

  • Well suited for LC-MS applications

Typical Applications

  • Ion exchange chromatography

  • Cation separations

  • Basic compound analysis

  • Pharmaceutical analysis

  • Amine separations

  • Peptide analysis

  • Protein analysis

  • USP L9 methods

  • LC-MS methods

  • Impurity profiling

  • Purification of positively charged compounds

Specifications

  • Product Type: HPLC Column

  • Stationary Phase: IEX WCX

  • USP Classification: L9

  • Chromatography Mode: Weak Cation Exchange

  • Functional Group: Carboxylic Acid

  • Particle Size: 5 µm

  • Pore Size: 100 Å

  • Column Dimensions: 2.1 mm ID x 150 mm

  • Length: 150 mm

  • Recommended Flow Rate: 0.2–0.3 mL/min

  • Brand: Cogent

Important Notes

  • Designed for weak cation exchange chromatography

  • Retains positively charged analytes

  • Functional charged range approximately pH 2.5–7

  • Suitable for many USP L9 applications

  • High-purity silica provides reliable chromatographic performance

  • Longer column length improves separation of closely related compounds and impurities

  • Excellent choice for LC-MS workflows

  • Standard HPLC systems must be capable of reliable low-flow operation for use with 2.1 mm ID columns


 

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